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- Host
- Human
- Application
- Western Blotting (WB)
- Characteristics
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: brain, 3: colon, 4: heart, 5: kidney, 6: liver, 7: lung, 8: pancreas, and 9: spleen.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Hamster Complement (Lyophilized) With Diluent
Host: Hamster Normal Complement
Guinea Pig Liver Acetone Tissue PowderIA Host: Guinea Pig Normal Liver
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- Application Notes
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
- Restrictions
- For Research Use only
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- Handling Advice
- The blot can be reused after stripping.
- Storage
- 4 °C
- Storage Comment
- Store at 4 °C.
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