Spectrin, Alpha, beta antibody
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- Target
- Spectrin, Alpha, beta
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Cross reactivity with Spectrin from other sources has not been determined.
- Purification
- Anti-Spectrin antibody was purified from mouse ascites by protein A chromatography. This product reacts with human.
- Immunogen
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Immunogen: Spectrin(alpha and beta) antibody was produced in mice by repeated immunizations with human erythrocyte spectrin.
Immunogen Type: Other
- Clone
- Spe 1-2
- Isotype
- IgG1
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- Application Notes
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Application Note: Anti-Spectrin is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~246-280 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:40,000
Western Blot Dilution: 2-4 μg/mL
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Neutral PBS
- Buffer
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- Spectrin, Alpha, beta
- Alternative Name
- Spectrin (alpha and beta)
- Background
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Synonyms: EL2, HPP, HS3, SPH3, SPTA, EL3, HS2, SPH2, HSPTB1, Erythroid alpha-spectrin
Background: Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5. This antibody is suitable for researchers interested in cytoskeleton and transcriptoion factor research.
Gene Name: SPTA1, SPTB
- Gene ID
- 6708
- NCBI Accession
- NP_003117
- UniProt
- P02549
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