Mouse anti-Human IgG Antibody
(1 reference)-
- Target See all IgG products
- IgG
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- ELISA, Western Blotting (WB)
- Purpose
- human IgG Antibody
- Characteristics
- Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
- Purification
- Purified antibody
- Immunogen
- Human IgG was isolated from human sera and purified by chromatography.
- Clone
- 4D2D9G8
- Isotype
- IgG1
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Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (F(ab')2 Region) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Mouse anti-Human IgG (Fab Region) antibodyELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Rabbit anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (Fc Region) antibody (HRP)ELISA, WB Polyclonal HRP F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (PE)ELISA, FACS Monoclonal JDC-10 IgG1 PE
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- Application Notes
- ELISA: 1/10000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified antibody in PBS containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).
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: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma
cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor. - Molecular Weight
- 50kDa
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