Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN965363

Product Details

Target: IgG

Fragment: Fab fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C

Storage Comment: Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid.

Expiry Date: 12 months

References

Dembla, Kesharwani, Natarajan, Fecher-Trost, Fairless, Williams, Flockerzi, Diem, Schwarz, Schmitz: "Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis." in: EMBO molecular medicine, Vol. 10, Issue 11, (2019) (PubMed).

Eich, Dembla, Wahl, Dembla, Schwarz, Schmitz: "The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System." in: Frontiers in molecular neuroscience, Vol. 10, pp. 230, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Fab Anti-Mouse IgG Antibody, Rabbit Fab Fragment Anti-Mouse IgG Antibody

Background: Fab Anti-Mouse IgG (H&L) Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.