Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN965310

Product Details

Target: IgG

Fragment: Fab fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Chicken IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Storage: RT,4 °C,-20 °C

Storage Comment: Store the vial at -20 °C or below after dilution.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Fab Anti-Chicken IgG Biotin Conjugated Antibody, Rabbit Fab Fragment Anti-Chicken IgG Antibody Biotin Conjugation

Background: Fab Anti-Chicken IgG Biotin Antibody generated in rabbit detects chicken IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.