Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN965308

Product Details

Target: IgG

Fragment: Fab fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum.

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Chicken IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.1

Application Details

Application Notes:

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Fab Anti-Chicken Antibody Fluorescein Conjugation, Rabbit Fab Anti-Chicken FITC Conjugated Antibody

Background: Fab Anti-Chicken IgG Fluorescein Antibody generated in rabbit detects chicken IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.