Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Sheep
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Sheep IgG whole molecule
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. - Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Donkey F(ab')2 Anti-Sheep IgG Biotin Conjugated Antibody, Donkey Fab2 Anti-Sheep IgG Antibody Biotin Conjugation
Background: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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