Goat anti-Rat IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN965245

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Fc Region

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum.

Cross-Reactivity: Rat (Rattus)

Characteristics: Anti-Rat Fc antibody generated in goat detects specifically rat IgG F(c). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Anti-Rat Fc antibody generated in goat detects specifically rat IgG F(c). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Rat IgG F(c) was produced by repeated immunization with Rat F(c) fragment in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:3,000

Application Note: Rat Fc secondary antibody is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:2,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 Anti-Rat IgG F(c) Antibody, Goat Fab2 Anti-Rat IgG Fc Antibody

Background: F(ab')2 Anti-Rat IgG F(c) Antibody was generated in goat and detects specifically Rat IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.