Goat anti-Rabbit IgG (F(ab')2 Region) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN965224

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: F(ab')2 Region

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Cross-Reactivity: Rabbit

Characteristics: F(ab')2 anti-Rabbit IgG antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: F(ab')2 anti-Rabbit IgG is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 Anti-Rabbit IgG biotin conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody biotin Conjugation

Background: F(ab')2 anti-Rabbit IgG antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.