Donkey anti-Mouse IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN965200

Supplier Product No.: 710-702-124

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Donkey

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 710-702-124

Supplier: Rockland

Purpose: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Characteristics: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Piccolella, Crippa, Cristofani, Rusmini, Galbiati, Cicardi, Meroni, Ferri, Morelli, Carra, Messi, Poletti: "The small heat shock protein B8 (HSPB8) modulates proliferation and migration of breast cancer cells." in: Oncotarget, Vol. 8, Issue 6, pp. 10400-10415, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.