Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN965171

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: Immunoassay (IA)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.

Characteristics: F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Preadsorption: Solid phase absorption

Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Immunogen Type: Native Protein

Application Details

Application Notes: F(ab') 2 anti-Mouse IgG (H&L) peroxidase conjugated antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Comment:

Post Translational Modification: Phosphorylation

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

References

Nakayama, Sakashita, Nariai, Kato, Sinmyozu, Nakayama, Kyo, Urano, Nakayama: "Cancer-related transcription regulator protein NAC1 forms a protein complex with CARM1 for ovarian cancer progression." in: Oncotarget, Vol. 9, Issue 47, pp. 28408-28420, (2018) (PubMed).

Tomiyama, Kamino, Fukamachi, Urano: "Precise epitope determination of the anti-vimentin monoclonal antibody V9." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 3917-3921, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: F(ab')2 Mouse IgG (H&L) Antibody, Gt-a-F(ab')2 Mouse IgG(H&L) Peroxidase conjugated, F(ab')2 Mouse IgG(H&L) antibody in goat, F(ab')2 Mouse IgG (H&L) Peroxidase conjugated Secondary Antibody.