Goat anti-Human IgG (F(ab')2 Region) Antibody - Preadsorbed

Catalog No. ABIN965093

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum.

Cross-Reactivity: Human

Characteristics: Anti-Human antibody generated in goat detects specifically human IgG F(ab')2. This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Anti-Human antibody generated in goat detects specifically human IgG F(ab')2. This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Human IgG was produced by repeated immunization with human IgG F(ab')2 fragment in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Antibody Anti-Human IgG F(ab')2 is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:25,000

Western Blot Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store vial antibody at 4 °C prior to restoration. For extended storage aliquot antibody and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

References

Chen, Williams: "Molecular mediators for raft-dependent endocytosis of syndecan-1, a highly conserved, multifunctional receptor." in: The Journal of biological chemistry, Vol. 288, Issue 20, pp. 13988-99, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody Pre-Adsorbed, Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody

Background: F(ab')2 Anti-Human IgG F(ab')2 Antibody generated in goat detects F(ab')2 from human. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.