Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN965012

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Golden Syrian Hamster, Armenian Hamster

Host: Goat

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Secondary AntibodyAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Golden Syrian Hamster IgG and Armenian Hamster IgG.

Characteristics: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian and Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Golden Syrian & Armenian Hamster IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Golden Syrian & Armenian Hamster IgG antibody, goat anti-Hamster IgG antibody

Background: Anti-Golden Syrian & Armenian Hamster IgG Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.