Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN964977

Supplier Product No.: 611-1322-0100

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 611-1322-0100

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Human Serum Proteins.

Characteristics: Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rabbit IgG F(c) will not bind rabbit IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG F(ab')2 is conjugated to Peroxidase.

Purification: Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Rabbit IgG whole molecule

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Goat Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:750 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:50,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

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Agrawal, Lehtonen, Uusi-Mäkelä, Jain, Viitala, Määttä, Kähkönen, Azizi, Riihimäki, Kulomaa, Johnson, Hytönen, Airenne: "Molecular features of steroid-binding antidins and their use for assaying serum progesterone." in: PLoS ONE, Vol. 14, Issue 2, pp. e0212339, (2019) (PubMed).

Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." in: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Millonig, Ganzleben, Peccerella, Casanovas, Brodziak-Jarosz, Breitkopf-Heinlein, Dick, Seitz, Muckenthaler, Mueller: "Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3)." in: The Journal of biological chemistry, Vol. 287, Issue 44, pp. 37472-82, (2013) (PubMed).

Grando, Bystryn, Chernyavsky, Frusić-Zlotkin, Gniadecki, Lotti, Milner, Pittelkow, Pincelli: "Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis." in: Experimental dermatology, Vol. 18, Issue 9, pp. 764-70, (2009) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG is conjugated with Peroxidase. This peroxidase conjugated anti-Rabbit secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.