Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN964977

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificity: IgG (H&L)

Characteristics: Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorbed

Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in goat.

Isotype: IgG

Application Details

Application Notes: Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. ELISA: 1:10,000-1:100,000 IHC: 1:500-1:5,000 WB: 1:5,000-1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: Lot specific

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 24 months

References

Cong, Gong, Yang, Xia, Zhang: "miR-22 Suppresses Tumor Invasion and Metastasis in Colorectal Cancer by Targeting NLRP3." in: Cancer management and research, Vol. 12, pp. 5419-5429, (2020) (PubMed).

Nuwer, Fleck: "Anterograde trafficking signals in GABAA subunits are required for functional expression." in: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).

Agrawal, Lehtonen, Uusi-Mäkelä, Jain, Viitala, Määttä, Kähkönen, Azizi, Riihimäki, Kulomaa, Johnson, Hytönen, Airenne: "Molecular features of steroid-binding antidins and their use for assaying serum progesterone." in: PLoS ONE, Vol. 14, Issue 2, pp. e0212339, (2019) (PubMed).

Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." in: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Millonig, Ganzleben, Peccerella, Casanovas, Brodziak-Jarosz, Breitkopf-Heinlein, Dick, Seitz, Muckenthaler, Mueller: "Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3)." in: The Journal of biological chemistry, Vol. 287, Issue 44, pp. 37472-82, (2013) (PubMed).

Grando, Bystryn, Chernyavsky, Frusić-Zlotkin, Gniadecki, Lotti, Milner, Pittelkow, Pincelli: "Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis." in: Experimental dermatology, Vol. 18, Issue 9, pp. 764-70, (2009) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Rabbit Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.