Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN964951

Supplier Product No.: 610-1319-0100

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 610-1319-0100

Supplier: Rockland

Purpose: Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Human Serum Proteins.

Characteristics: Anti-Mouse IgM (mu chain) peroxidase conjugated antibody generated in goat detects specifically mouse IgM.

Purification: HRP Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Mouse secondary antibody conjugated to peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for Immunohistochemistry, Western blotting as well as other antibody based assays. Immunohistochemistry Dilution: 1:1,000 - 1:8,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:20,000 - 1:80,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

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Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." in: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Castaño, Caviedes, Hirsch, Llanos, Iñiguez, Ronco: "Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels." in: PloS one, Vol. 12, Issue 1, pp. e0170389, (2017) (PubMed).

DLima, Ma, Winkler, Chu, Loh, Corpuz, Budnik, Lykke-Andersen, Saghatelian, Slavoff: "A human microprotein that interacts with the mRNA decapping complex." in: Nature chemical biology, Vol. 13, Issue 2, pp. 174-180, (2016) (PubMed).

Ray, Chopra, Long, Lahiri: "Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research." in: Molecular brain, Vol. 7, pp. 63, (2015) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Frasca, Romero, Diaz, Alter-Wolf, Ratliff, Landin, Riley, Blomberg: "A molecular mechanism for TNF-α-mediated downregulation of B cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 1, pp. 279-86, (2012) (PubMed).

Frasca, Romero, Landin, Diaz, Riley, Blomberg: "Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability." in: Mechanisms of ageing and development, Vol. 131, Issue 5, pp. 306-14, (2010) (PubMed).

Ronsyn, Daans, Spaepen, Chatterjee, Vermeulen, DHaese, Van Tendeloo, Van Marck, Ysebaert, Berneman, Jorens, Ponsaerts: "Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord." in: BMC biotechnology, Vol. 7, pp. 90, (2008) (PubMed).

Williams, Weichert, Zakrzewicz, Da Silva-Azevedo, Pries, Baum, Egginton: "Differential gene and protein expression in abluminal sprouting and intraluminal splitting forms of angiogenesis." in: Clinical science (London, England : 1979), Vol. 110, Issue 5, pp. 587-95, (2006) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.