Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
- IgG (H&L)
- Cross-Reactivity
- Mouse (Murine)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Anti-Mouse IgG (H&L) was produced by repeated immunization with mouse IgG whole molecule in goat.
Immunogen Type: Native Protein
- Isotype
- IgG
- Labeling Ratio
- 3.6
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- Application Notes
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Application Note: Anti-Mouse IgG FITC Conjugated Antibody is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: User Optimized
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Goat Anti-Mouse IgG Secondary Antibody fluorescein Conjugated, Goat Anti-Mouse IgG Antibody FITC Conjugated, GAM-FITC, Anti-mouse IgG secondary antibody, anti-mouse IgG Fluorescein conjugated secondary antibody, Gt Anti-Ms IgG FITC Conjugated Antibody
Background: Anti-Mouse IgG [H&L] Fluorescein Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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