Donkey anti-Donkey IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

Catalog No. ABIN964943

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat, Donkey

Host: Donkey

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Specificity: Goat IgG antibody recognizes IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum.

Characteristics: Anti-Goat IgG (H&L) antibody is ideal for investigators involved in serum component protein research.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Goat IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Goat IgG (H&L) antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.

Storage: RT,4 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.

Expiry Date: 6 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Donkey anti-Goat IgG Antibody Phycoerythrin Conjugation, Donkey anti-Goat IgG PE Conjugated Antibody

Background: Goat IgG antibody recognizes IgG heavy and light chains. Anti-Goat IgG Phycoerythrin Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Goat IgG (H&L) antibody is ideal for investigators involved in serum component protein research.