Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN964937

Supplier Product No.: 605-4313-0100

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 605-4313-0100

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.

Characteristics: Anti-Goat IgG F(ab')2 Peroxidase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Goat IgG whole molecule

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Goat IgG (H&L) peroxidase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:20,000 - 1:120,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Ercan, Han, Di Nardo, Winden, Han, Hoyo, Saffari, Leask, Geschwind, Sahin: "Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex." in: The Journal of experimental medicine, Vol. 214, Issue 3, pp. 681-697, (2017) (PubMed).

Ko, Ko, Shieh, Chi, Chen, Chen, Yu, Yang, Chang: "Advanced glycation end products influence oral cancer cell survival via Bcl-xl and Nrf-2 regulation in vitro." in: Oncology letters, Vol. 13, Issue 5, pp. 3328-3334, (2017) (PubMed).

van der Hoorn, de Haan, Berbée, Havekes, Jukema, Rensen, Princen: "Niacin increases HDL by reducing hepatic expression and plasma levels of cholesteryl ester transfer protein in APOE*3Leiden.CETP mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 11, pp. 2016-22, (2008) (PubMed).

van der Hoogt, de Haan, Westerterp, Hoekstra, Dallinga-Thie, Romijn, Princen, Jukema, Havekes, Rensen: "Fenofibrate increases HDL-cholesterol by reducing cholesteryl ester transfer protein expression." in: Journal of lipid research, Vol. 48, Issue 8, pp. 1763-71, (2007) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.