Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN964937

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificity: IgG (H&L)

Characteristics: Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Goat IgG (h&L) was produced by repeated immunization with goat IgG whole molecule in rabbit.

Isotype: IgG

Application Details

Application Notes: Antibody Anti-Goat IgG (H&L) peroxidase conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Ercan, Han, Di Nardo, Winden, Han, Hoyo, Saffari, Leask, Geschwind, Sahin: "Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex." in: The Journal of experimental medicine, Vol. 214, Issue 3, pp. 681-697, (2017) (PubMed).

Ko, Ko, Shieh, Chi, Chen, Chen, Yu, Yang, Chang: "Advanced glycation end products influence oral cancer cell survival via Bcl-xl and Nrf-2 regulation in vitro." in: Oncology letters, Vol. 13, Issue 5, pp. 3328-3334, (2017) (PubMed).

van der Hoorn, de Haan, Berbée, Havekes, Jukema, Rensen, Princen: "Niacin increases HDL by reducing hepatic expression and plasma levels of cholesteryl ester transfer protein in APOE*3Leiden.CETP mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 11, pp. 2016-22, (2008) (PubMed).

van der Hoogt, de Haan, Westerterp, Hoekstra, Dallinga-Thie, Romijn, Princen, Jukema, Havekes, Rensen: "Fenofibrate increases HDL-cholesterol by reducing cholesteryl ester transfer protein expression." in: Journal of lipid research, Vol. 48, Issue 8, pp. 1763-71, (2007) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Goat IgG (H&L) Antibody, Rb-a-Goat IgG (H&L) Peroxidase conjugated, Goat IgG (H&L) Peroxidase Antibody in rabbit, Rabbit IgG(H&L) peroxidase conjugated Secondary Antibody.