Mouse anti-Cow Ig Light Chains Antibody

Catalog No. ABIN94401

Product Details

Target: Ig Light Chains

Reactivity: Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Specificity: The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

Cross-Reactivity (Details): Bovine, Sheep

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Fraction of Bovine immunoglobulin.

Clone: IVA285-1

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).

Target Details

Target: Ig Light Chains

Background: Immunoglobulin light chains