Goat anti-Chimpanzee IgA (Free & Bound), (Secretory Component) Antibody (FITC)
WB, IF, IHC (fro), ID, IP, EIA, DB
Polyclonal
IgG
FITC
-
- Target See all IgA products
- IgA
-
Binding Specificity
- Free & Bound, Secretory Component
-
Reactivity
- Monkey, Chimpanzee
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- FITC
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)
- Cross-Reactivity
- Monkey
- Characteristics
- Molar Ratio: 1,6
- Purification
- DEAE-column Chromatography
- Immunogen
- Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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-
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
-
Absorption / Emission: 492 nm/512 nm
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore with 1 mL sterile distilled water
- Concentration
- 10 mg/mL
- Buffer
- PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
-
Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. - Storage
- 4 °C/-20 °C
- Storage Comment
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
-
- Target
- IgA
- Abstract
- IgA Products
- Target Type
- Antibody
- Background
- Synonyms: Monkey Immunoglobulin A
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