Rabbit anti-Llama VHH Antibody
ELISA, WB
Polyclonal
IgG
unconjugated
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- Target
- VHH
- Reactivity
- Llama
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Host
- Rabbit
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Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Purpose
- Rabbit Anti-VHH antibody can be used to detect single domain VHH antibodies.
- Cross-Reactivity (Details)
- Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.
- Characteristics
- Anti-ALG6 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase is an enzyme that is encoded by the ALG6 gene in humans. This gene encodes ALG6/ALG8 member of glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type. Anti-ALG6 is ideal for researchers interested in Infectious disease, especially HIV and hepatitis virus infections. Anti-ALG6 antibody is applicable to methods for diagnosis and, in particular, to methods for diagnosing infections using biomarkers targeting exosomes secreted in bodily fluids.
- Purification
- Anti-VHH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-VHH antibody has been tested by ELISA and Western blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed. Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:10,000-1:50,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 80 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- VHH
- Background
- The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VHH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VHH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.
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