Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (FITC)

Catalog No. ABIN7539591

Supplier Product No.: 611-1202-1.0

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Multiplex Assay (MA)

Supplier Product No.: 611-1202-1.0

Supplier: Rockland

Purpose: Goat Anti-Rabbit IgG H&L (FITC)

Specificity: IgG (H&L)

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Grade: High-quality product from Rockland

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Anti-Rabbit IgG Antibody Fluorescein has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

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Gupta, Lorentz, Haskett, Cunnane, Ramaswamy, Weinbaum, Vorp, Mandal: "Bioresorbable silk grafts for small diameter vascular tissue engineering applications: In vitro and in vivo functional analysis." in: Acta biomaterialia, Vol. 105, pp. 146-158, (2021) (PubMed).

Wang, Chen, Li, Zhang, Guo, Hu, Shao, Song, Zhao, Li, Yang, Xu, Wei: "Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor." in: Redox biology, Vol. 32, pp. 101514, (2021) (PubMed).

Cao, Zhang, Du, Liu, Qiu, Peng: "TGF-β1 Provides Neuroprotection via Inhibition of Microglial Activation in 3-Acetylpyridine-Induced Cerebellar Ataxia Model Rats." in: Frontiers in neuroscience, Vol. 14, pp. 187, (2020) (PubMed).

Haskett, Saleh, Lorentz, Josowitz, Luketich, Weinbaum, Kokai, DAmore, Marra, Rubin, Wagner, Vorp: "An exploratory study on the preparation and evaluation of a "same-day" adipose stem cell-based tissue-engineered vascular graft." in: The Journal of thoracic and cardiovascular surgery, Vol. 156, Issue 5, pp. 1814-1822.e3, (2019) (PubMed).

Cuoghi, Lazzaretti, Mandrioli, Mola, Pederzoli: "Immunohistochemical analysis of the distribution of molecules involved in ionic and pH regulation in the lancelet Branchiostoma floridae (Hubbs, 1922)." in: Acta histochemica, Vol. 120, Issue 1, pp. 33-40, (2018) (PubMed).

Li, Wu, Yang, Liu, Chen: "Sesquiterpene lactone 6-O-angeloylplenolin reverses vincristine resistance by inhibiting YB-1 nuclear translocation in colon carcinoma cells." in: Oncology letters, Vol. 15, Issue 6, pp. 9673-9680, (2018) (PubMed).

Vitacolonna, Belharazem, Hohenberger, Roessner: "In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue." in: Cell and tissue banking, Vol. 18, Issue 1, pp. 27-43, (2017) (PubMed).

Krawiec, Liao, Kwan, DAmore, Weinbaum, Rubin, Wagner, Vorp: "Evaluation of the stromal vascular fraction of adipose tissue as the basis for a stem cell-based tissue-engineered vascular graft." in: Journal of vascular surgery, Vol. 66, Issue 3, pp. 883-890.e1, (2017) (PubMed).

Krawiec, Weinbaum, Liao, Ramaswamy, Pezzone, Josowitz, DAmore, Rubin, Wagner, Vorp: "In Vivo Functional Evaluation of Tissue-Engineered Vascular Grafts Fabricated Using Human Adipose-Derived Stem Cells from High Cardiovascular Risk Populations." in: Tissue engineering. Part A, Vol. 22, Issue 9-10, pp. 765-75, (2017) (PubMed).

Xu, Xu, Wang, Zhang: "Lefty1 alleviates renal tubulointerstitial injury in mice with unilateral ureteral obstruction." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 901-8, (2016) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1