Rabbit anti-Cow IgG (Heavy & Light Chain) Antibody (HRP)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA
- Supplier Product No.
- 601-4302-1.0
- Supplier
- Rockland
- Purpose
- Rabbit Anti-Cow IgG H&L (HRP)
- Specificity
- IgG (H&L)
- Purification
- Anti-Bovine IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
- Grade
- High-quality product from Rockland
- Immunogen
- Bovine IgG whole molecule
- Isotype
- IgG
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- Application Notes
- Anti-Bovine IgG Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/ml Polyethylene Glycol (PEG-8000)
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
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