Rabbit anti-Rat IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN7539588

Supplier Product No.: 612-4302-1.0

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 612-4302-1.0

Supplier: Rockland

Purpose: Rabbit Anti-Rat IgG H&L (HRP)

Specificity: IgG (H&L)

Purification: Secondary Antibody Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum.

Grade: High-quality product from Rockland

Immunogen: Rat IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Anti-Rat IgG Peroxidase conjugated antibody has been tested by ELISA and is suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Blair, Liu, Labitigan, Wu, Zheng, Xia, Pearson, Nazeer, Cao, Lang, Rines, Mackintosh, Moore, Li, Tian, Tackett, Yan: "KDM5 lysine demethylases are involved in maintenance of 3'UTR length." in: Science advances, Vol. 2, Issue 11, pp. e1501662, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1