Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN7539586

Supplier Product No.: 610-4302-1.0

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Supplier Product No.: 610-4302-1.0

Supplier: Rockland

Purpose: Rabbit Anti-Mouse IgG H&L (HRP)

Specificity: IgG (H&L)

Purification: HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Grade: High-quality product from Rockland

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

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Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1