Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN7539583

Supplier Product No.: 611-1302-1.0

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 611-1302-1.0

Supplier: Rockland

Purpose: Goat Anti-Rabbit IgG H&L (HRP)

Specificity: IgG (H&L)

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Grade: High-quality product from Rockland

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Mochizuki, Okumo, Takemura, Izukashi, Tatsuo, Ikemoto, Adachi, Kawate, Sunagawa: "Suppressive Activity of Boiogito, a Japanese Traditional Kampo Medicine, on Periostin Secretion in Human Fibroblast-Like Synoviocytes In Vitro." in: Cureus, Vol. 16, Issue 4, pp. e57690, (2024) (PubMed).

He, Wu, Sun, Yuan, Hu, Yang, Chen, Yan, Yin, Zhang, Li: "Functional assessment of a novel biallelic MYH3 variation causing CPSKF1B (contractures, pterygia, and spondylocarpotarsal fusion syndrome1B)." in: Molecular genetics & genomic medicine, Vol. 12, Issue 3, pp. e2401, (2024) (PubMed).

Guo, Sperber, Krieger, Duan, Chemelewski, Sacchettini, Herman: "Bacillus subtilis YisK possesses oxaloacetate decarboxylase activity and exhibits Mbl-dependent localization." in: Journal of bacteriology, Vol. 206, Issue 1, pp. e0020223, (2024) (PubMed).

Traczyk, Hromada-Judycka, Świątkowska, Wiśniewska, Ciesielska, Kwiatkowska: "Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment." in: Journal of lipid research, Vol. 65, Issue 1, pp. 100480, (2024) (PubMed).

van Soest, Polderman, den Toom, Keijer, van Roosmalen, Leyten, Lehmann, Zwakenberg, De Henau, van Boxtel, Burgering, Dansen: "Mitochondrial H2O2 release does not directly cause damage to chromosomal DNA." in: Nature communications, Vol. 15, Issue 1, pp. 2725, (2024) (PubMed).

Kücük, Abbey, Schmitt, Henninger, Fritz: "Cardiomyocytes, cardiac endothelial cells and fibroblasts contribute to anthracycline-induced cardiac injury through RAS-homologous small GTPases RAC1 and CDC42." in: Pharmacological research, Vol. 203, pp. 107165, (2024) (PubMed).

Schröder, Wagner, Cieplik, Spanier, Proff, Kirschneck: "Impact of phosphorylation of heat shock protein 27 on the expression profile of periodontal ligament fibroblasts during mechanical strain." in: Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, Vol. 84, Issue Suppl 2, pp. 143-153, (2023) (PubMed).

Dai, Park, Du, Na, Lam, Chow, Renauer, Gu, Xin, Chu, Liao, Clark, Zhao, Slavoff, Chen: "Massively parallel knock-in engineering of human T cells." in: Nature biotechnology, Vol. 41, Issue 9, pp. 1239-1255, (2023) (PubMed).

Arczewska-Włosek, Świątkiewicz, Tomaszewska, Muszyński, Dobrowolski, Józefiak: "Effects of Anticoccidial Vaccination and Taraxacum officinale Extract on the Growth Performance, Biochemical Parameters, Immunity, and Intestinal Morphology of Eimeria-Challenged Chickens." in: Life (Basel, Switzerland), Vol. 13, Issue 9, (2023) (PubMed).

Bunduc, Ding, Kuijl, Marlovits, Bitter, Houben: "Reconstitution of a minimal ESX-5 type VII secretion system suggests a role for PPE proteins in the outer membrane transport of proteins." in: mSphere, Vol. 8, Issue 5, pp. e0040223, (2023) (PubMed).

Park, Miyamoto, Han, Chan, Curnutt, Tran, Velleca, Kim, Schurer, Chang, Xu, Kharas, Woo: "Dual IKZF2 and CK1α degrader targets acute myeloid leukemia cells." in: Cancer cell, Vol. 41, Issue 4, pp. 726-739.e11, (2023) (PubMed).

ONeill, Anderson, Mukherjee, Gandu, McEwan, Omelchenko, Rodriguez, Losert, Meaney, Babadi, Firestein: "Time-dependent homeostatic mechanisms underlie brain-derived neurotrophic factor action on neural circuitry." in: Communications biology, Vol. 6, Issue 1, pp. 1278, (2023) (PubMed).

Castro, Umana-Perez, Castaño-Moreno, Casanello, Ronco: "DHA Supplementation during Pregnancy in Women with Obesity Normalizes IGF2R Levels in the Placenta of Male Newborns." in: International journal of endocrinology, Vol. 2023, pp. 1515033, (2023) (PubMed).

Chen, Su, Zhao, Na, Jiang, Bacchiocchi, Loh, Halaban, Wang, Cao, Slavoff: "Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5." in: Cell reports, Vol. 42, Issue 9, pp. 113145, (2023) (PubMed).

Liu, Samant, Vasa, Pedrigi, Oguz, Ryu, Wei, Anderson, Agrawal, Chatzizisis: "Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis." in: PloS one, Vol. 18, Issue 1, pp. e0280385, (2023) (PubMed).

Bina, Hines, Sanyal, Taneyhill: "Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches." in: Journal of developmental biology, Vol. 11, Issue 1, (2023) (PubMed).

Halmi, Wu, Taneyhill: "Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly." in: F1000Research, Vol. 11, pp. 741, (2023) (PubMed).

Küchler, Teodoro, Schröder, Nazet, Meger, Kunz, Baratto-Filho, Spanier, Scariot, Proff, Kirschneck: "Effect of genetic polymorphisms rs2301113 and rs2057482 in the expression of HIF-1α protein in periodontal ligament fibroblasts subjected to compressive force." in: Journal of applied oral science : revista FOB, Vol. 31, pp. e20220151, (2023) (PubMed).

Timón-Gómez, Pérez-Pérez, Nyvltova, Ugalde, Fontanesi, Barrientos: "Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis." in: STAR protocols, Vol. 1, Issue 2, (2022) (PubMed).

Ramirez, Yang, DSouza, Shen, Woo: "Truncation of the TPR domain of OGT alters substrate and glycosite selection." in: Analytical and bioanalytical chemistry, Vol. 413, Issue 30, pp. 7385-7399, (2022) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1