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Recombinant Alpaca anti-Mouse IgG1 Antibody (Atto 488)

WB, IHC, FACS, IF, ICC Monoclonal 10A4 Recombinant Antibody Atto 488 single-domain Antibody (sdAb)

Catalog No. ABIN7272921

Datasheet as PDF

Target See all IgG1 products

IgG1

Antibody Type

Recombinant Antibody

Fragment

single-domain Antibody (sdAb)
Reactivity
94

24

23

9

7

5

4

4

3

2

1
Mouse
Host
61

42

39

15

10

5

1
Alpaca

Expression System

E.coli
Clonality
93

59
Monoclonal
Conjugate
46

21

21

18

11

8

7

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Atto 488
Application
90

59

50

45

30

19

18

15

14

11

10

9

8

6

4

3

2

2

2
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity

Mouse IgG1, does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Purification

Produced in: E.coli

Immunogen

FluoTag-X2 anti-Mouse IgG1 is a species-specific FluoTag directed against IgG1 from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG1 sdAbs bind specifically and strongly to mouse IgG1 independently of the IgGs light chains.

Clone

10A4

Labeling Ratio

Two site-specifically conjugated fluorophores per sdAb.
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Rat anti-Mouse IgG1 antibody
ELISA, FACS Monoclonal SB77e IgG2b unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)
IHC, FACS Polyclonal IgG Cy5

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

Application Notes

1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer

The single sdAb clone was lyophilized from PBS pH 7.4.

Storage

-20 °C/-80 °C

Storage Comment

Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
Target

IgG1

Abstract

IgG1 Products

Target Type

Antibody

Background

In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.