Recombinant Mouse anti-Human IgG Antibody (HRP)
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- Target See all IgG products
- IgG
- Antibody Type
- Recombinant Antibody
- Fragment
- scFv fragment
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- HRP
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Application
- ELISA, Western Blotting (WB), Dot Blot (DB)
- Purpose
- Anti-Human IgG scFv-HRP Antibody
- Specificity
- Mouse variable regions (produced in Nicotiana benthamiana plants)
- Characteristics
- This recombinantly produced secondary anti-human IgG scFv fused to HRP is optimised for western blot and ELISA use. The reddish/brown colour is common with HRP conjugated antibodies.
- Purification
- This product was purified using affinity chromatography (Ni-IMAC)
- Purity
- 90-95 %
- Immunogen
- Human IgG, whole molecule
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Fc Region) antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS Polyclonal IgG Texas Red (TR)
Goat anti-Human IgG antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
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- Application Notes
- Western blot: Suggested working dilution 1/1000-1/5000 to detect 1 μg antigen in 5 % milk. Suggested incubation conditions:1 hr at 37C/ 4C overnight.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate Buffered saline (0.1M) pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 2-4°C. Long term: -20°C
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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