Rabbit anti-Pig IgG Antibody (DyLight 800) - Preadsorbed

Catalog No. ABIN6699196

Product Details

Target: IgG

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: DyLight 800

Application: ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Characteristics:

Synonyms: Rabbit anti-Swine Antibody, Anti-Pig IgG (H&L) (Rabbit), Swine Secondary Antibody, Swine in Rabbit Ab

Background: SWINE IgG (H&L) (RABBIT) Antibody generated in rabbit detects specifically swine IgG whole molecule. Anti-Swine IgG antibody is ideal for investigators involved in serum protein component research.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Swine IgG whole molecule

Isotype: IgG

Labeling Ratio: 1.9

Application Details

Application Notes:

Application Note: Anti-SWINE IgG (H&L) (RABBIT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Specific conditions should be optimized by user.

Immunoprecipitation Dilution: 1:1,000 - 1:5,000

FLISA Dilution: >1:20,000

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody