Goat anti-Rabbit IgG (Fc Region) Antibody (DyLight 800)

Catalog No. ABIN6699130

Supplier Product No.: 611-145-003

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 611-145-003

Supplier: Rockland

Purpose: Rabbit IgG Fc Antibody DyLight™ 800 Conjugated

Characteristics: Goat Anti Rabbit IgG F(c) DyLight 800™ Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody DyLight 800™ conjugation, Goat Anti Rabbit IgG Fc Antibody DyLight 800™ conjugated,Anti-Rabbit IgG F(c) DyLight generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Immunogen: Rabbit IgG F(c) fragment

Isotype: IgG

Labeling Ratio: 2.2

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Rabbit IgG F(c) DyLight ™ 800 conjugate has been tested by dot blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Anderson, Sastalla, Earland, Mahnaz, Moore, Otaizo-Carrasquero, Myers, Myles, Datta, Myles: "Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632." in: PLoS ONE, Vol. 13, Issue 9, pp. e0198862, (2019) (PubMed).

Kohnz, Roberts, DeTomaso, Bideyan, Yan, Bandyopadhyay, Goga, Yosef, Nomura: "Protein Sialylation Regulates a Gene Expression Signature that Promotes Breast Cancer Cell Pathogenicity." in: ACS chemical biology, Vol. 11, Issue 8, pp. 2131-9, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.