Goat anti-Rabbit IgG Antibody (DyLight 800) - Preadsorbed

Catalog No. ABIN6699120

Supplier Product No.: 611-145-122

Product Details

Target: IgG

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 611-145-122

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Characteristics: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody,Conjugated DyLight™ 800 secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.5

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Rabbit IgG DyLight™800 has been tested by dot blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: Other, WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Pereira, Bacman, Arguello, Zekonyte, Williams, Edgell, Moraes: "mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels." in: EMBO molecular medicine, Vol. 10, Issue 9, (2018) (PubMed).

Nguyen, Williams, Young, Ma, Li, Levis, Brown, Small: "FLT3 activating mutations display differential sensitivity to multiple tyrosine kinase inhibitors." in: Oncotarget, Vol. 8, Issue 7, pp. 10931-10944, (2017) (PubMed).

Meyer, Neeley, Baker, Washabaugh, Flesher, Nelson, Frankel, Lumeng, Lyssiotis, Wynn, Rhim, ORourke: "Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer." in: Biochemistry and biophysics reports, Vol. 7, pp. 144-149, (2016) (PubMed).

Klammer, Kaminski, Zedler, Oppermann, Blencke, Marx, Müller, Tebbe, Godl, Schaab: "Phosphosignature predicts dasatinib response in non-small cell lung cancer." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 9, pp. 651-68, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody