Goat anti-Rabbit IgG Antibody (DyLight 800) - Preadsorbed

Catalog No. ABIN6699120

Product Details

Target: IgG

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Characteristics:

Synonyms: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody

Background: Conjugated DyLight™ 800 secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.5

Application Details

Application Notes:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Pereira, Bacman, Arguello, Zekonyte, Williams, Edgell, Moraes: "mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels." in: EMBO molecular medicine, Vol. 10, Issue 9, (2018) (PubMed).

Nguyen, Williams, Young, Ma, Li, Levis, Brown, Small: "FLT3 activating mutations display differential sensitivity to multiple tyrosine kinase inhibitors." in: Oncotarget, Vol. 8, Issue 7, pp. 10931-10944, (2017) (PubMed).

Meyer, Neeley, Baker, Washabaugh, Flesher, Nelson, Frankel, Lumeng, Lyssiotis, Wynn, Rhim, ORourke: "Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer." in: Biochemistry and biophysics reports, Vol. 7, pp. 144-149, (2016) (PubMed).

Klammer, Kaminski, Zedler, Oppermann, Blencke, Marx, Müller, Tebbe, Godl, Schaab: "Phosphosignature predicts dasatinib response in non-small cell lung cancer." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 9, pp. 651-68, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody