Donkey anti-Rabbit IgG Antibody (DyLight 649) - Preadsorbed

Catalog No. ABIN6699111

Supplier Product No.: 611-743-127

Product Details

Target: IgG

Reactivity: Rabbit

Host: Donkey

Clonality: Polyclonal

Conjugate: DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 611-743-127

Supplier: Rockland

Purpose: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Characteristics: Donkey Anti-Rabbit IgG Antibody DyLight 649™ Conjugated, Donkey Anti Rabbit IgG DyLight 649™ Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 649 Antibody generated in donkey detects reactivity to Rabbit IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Labeling Ratio: 3.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Rabbit IgG (H&L) DyLight 649 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, Multiplex

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Ayyar, Ettayebi, Salmen, Karandikar, Neill, Tenge, Crawford, Bieberich, Prasad, Atmar, Estes: "CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection." in: Nature communications, Vol. 14, Issue 1, pp. 1148, (2023) (PubMed).

Onoda, Hagiwara, Kubota, Yanagita, Takeda, Umezawa: "A Novel Staining Method for Detection of Brain Perivascular Injuries Induced by Nanoparticle: Periodic Acid-Schiff and Immunohistochemical Double-Staining." in: Frontiers in toxicology, Vol. 4, pp. 825984, (2022) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Carbon nanoparticles induce endoplasmic reticulum stress around blood vessels with accumulation of misfolded proteins in the developing brain of offspring." in: Scientific reports, Vol. 10, Issue 1, pp. 10028, (2020) (PubMed).

Krzisch, Fülling, Jabinet, Armida, Gebara, Cassé, Habbas, Volterra, Hornung, Toni: "Synaptic Adhesion Molecules Regulate the Integration of New Granule Neurons in the Postnatal Mouse Hippocampus and their Impact on Spatial Memory." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 8, pp. 4048-4059, (2018) (PubMed).

Lee, Nevo: "Microvascular endothelial cells from preeclamptic women exhibit altered expression of angiogenic and vasopressor factors." in: American journal of physiology. Heart and circulatory physiology, Vol. 310, Issue 11, pp. H1834-41, (2017) (PubMed).

Tanase, Yokoo, Matsumura, Kinoshita, Kikuchi, Suemori, Ohyama: "Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm." in: Biochemical and biophysical research communications, Vol. 482, Issue 4, pp. 764-770, (2017) (PubMed).

Onoda, Takeda, Umezawa: "Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 4, (2017) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles." in: Frontiers in cellular neuroscience, Vol. 11, pp. 92, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.