Donkey anti-Rabbit IgG Antibody (DyLight 549) - Preadsorbed

Catalog No. ABIN6699106

Product Details

Target: IgG

Reactivity: Rabbit

Host: Donkey

Clonality: Polyclonal

Conjugate: DyLight 549

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Characteristics:

Synonyms: Donkey Anti-Rabbit IgG Antibody DyLight 549™ Conjugated, Donkey Anti Rabbit IgG DyLight 549™ Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) DyLight 549 Antibody generated in donkey detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Labeling Ratio: 3.3

Application Details

Application Notes:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Rabbit IgG (H&L) DyLight 549 Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Wang, Wang, Liu, Huang, Liu, Xia, Brecha, Pu, Gao: "Protective Effect of ALA in Crushed Optic Nerve Cat Retinal Ganglion Cells Using a New Marker RBPMS." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160309, (2017) (PubMed).

Ramamoorthy, Shi: "Ischemia induces different levels of hypoxia inducible factor-1α protein expression in interneurons and pyramidal neurons." in: Acta neuropathologica communications, Vol. 2, pp. 51, (2014) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody