Goat anti-Rabbit IgG Antibody (Cy2) - Preadsorbed

Catalog No. ABIN6699089

Product Details

Target: IgG

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: Cy2

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Characteristics:

Synonyms: Goat Anti Rabbit IgG Antibody CY2 Conjugation, Goat Anti-Rabbit IgG CY2 Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) Antibody CY2 Conjugated generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rabbit IgG whole molecule

Isotype: IgG

Labeling Ratio: 4.5

Application Details

Application Notes:

Application Note: Anti-Rabbit IgG (H&L) Conjugated Cy2 Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

ELISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

Western Blot Dilution: User Optimized

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store Cy2 Conjugated Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Cy2 secondary antibody is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References

Renko, Tolonen, Rysä, Magga, Mustonen, Ruskoaho, Serpi: "SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart." in: Scientific reports, Vol. 8, Issue 1, pp. 1160, (2018) (PubMed).

Venkatesan, Natarajan, Schwarz, Mayer, Alpadi, Magupalli, Sung, Schmitz: "Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 19, pp. 6559-76, (2010) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody