Rabbit anti-Mouse IgG3 (Heavy Chain) Antibody (DyLight 549)

Catalog No. ABIN6699079

Supplier Product No.: 610-442-043

Product Details

Target: IgG3

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: DyLight 549

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 610-442-043

Supplier: Rockland

Purpose: Mouse IgG3 (Gamma 3 chain) Antibody DyLight™ 549 Conjugated

Characteristics: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) DyLight 549™ Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody DyLight 549™ Conjugation,Anti-Mouse IgG3 DyLight 549 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain).

Immunogen: Mouse IgG3 heavy chain

Isotype: IgG

Labeling Ratio: 3.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Baumann, Bakkers, Buettner, Hartmann, Grove, Langereis, de Groot, Mühlenhoff: "9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate." in: Nature communications, Vol. 6, pp. 7673, (2016) (PubMed).

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.