Rabbit anti-Mouse IgG1 (Heavy Chain) Antibody (DyLight 649)

Catalog No. ABIN6699057

Supplier Product No.: 610-443-040

Product Details

Target: IgG1

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 610-443-040

Supplier: Rockland

Purpose: Mouse IgG1 (Gamma 1 chain) Antibody DyLight™ 649 Conjugated

Characteristics: Rabbit Anti Mouse IgG1 (Gamma 1 chain) Antibody DyLight 649™ Conjugated, Rabbit Anti-Mouse IgG1 Antibody DyLight 649™ Conjugated,Anti-Mouse IgG1 DyLight 649 Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain).

Immunogen: Mouse IgG1 heavy chain

Isotype: IgG

Labeling Ratio: 3.2

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: Microarray

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

White, Couetil, Richard, Marti, Wilson: "Microarray molecular mapping of horses with severe asthma." in: Journal of veterinary internal medicine, Vol. 38, Issue 1, pp. 477-484, (2024) (PubMed).

Wyler, Sage, Marti, White, Gerber: "Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma." in: Journal of veterinary internal medicine, Vol. 37, Issue 1, pp. 328-337, (2023) (PubMed).

Birras, White, Jonsdottir, Novotny, Ziegler, Wilson, Frey, Torsteinsdottir, Alcocer, Marti: "First clinical expression of equine insect bite hypersensitivity is associated with co-sensitization to multiple Culicoides allergens." in: PloS one, Vol. 16, Issue 11, pp. e0257819, (2021) (PubMed).

Novotny, White, Wilson, Stefánsdóttir, Tijhaar, Jonsdóttir, Frey, Reiche, Rose, Rhyner, Schüpbach-Regula, Torsteinsdóttir, Alcocer, Marti: "Component-resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity." in: Allergy, Vol. 76, Issue 4, pp. 1147-1157, (2021) (PubMed).

White, Moore-Colyer, Marti, Hannant, Gerber, Coüetil, Richard, Alcocer: "Antigen array for serological diagnosis and novel allergen identification in severe equine asthma." in: Scientific reports, Vol. 9, Issue 1, pp. 15170, (2020) (PubMed).

White, Moore-Colyer, Marti, Coüetil, Hannant, Richard, Alcocer: "Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma." in: Journal of veterinary internal medicine, Vol. 33, Issue 5, pp. 2327-2335, (2020) (PubMed).

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.