Goat anti-Mouse IgG Antibody (DyLight 800) - Preadsorbed

Catalog No. ABIN6699041

Product Details

Target: IgG

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Characteristics:

Synonyms: Goat Anti-Mouse IgG Secondary Antibody DyLight™800 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™800 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™800 conjugated secondary antibody

Background: Anti-Mouse IgG DyLight 800 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.4

Application Details

Application Notes:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

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Cao, Xue, Cheng, Wang, Liu, Li, Jiang, Li, Gui, Zhang: "MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1." in: Oncogene, Vol. 38, Issue 24, pp. 4835-4855, (2019) (PubMed).

Compte, Harwood, Muñoz, Navarro, Zonca, Perez-Chacon, Erce-Llamazares, Merino, Tapia-Galisteo, Cuesta, Mikkelsen, Caleiras, Nuñez-Prado, Aznar, Lykkemark, Martínez-Torrecuadrada, Melero, Blanco et al.: "A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. ..." in: Nature communications, Vol. 9, Issue 1, pp. 4809, (2018) (PubMed).

Lin, Chen, Wang, Cai: "Emodin promotes the arrest of human lymphoma Raji cell proliferation through the UHRF1‑DNMT3A‑∆Np73 pathways." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 6544-6551, (2018) (PubMed).

Binek, Fernández-Jiménez, Jorge, Camafeita, López, Bagwan, Galán-Arriola, Pun, Agüero, Fuster, Ibanez, Vázquez: "Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model." in: Scientific reports, Vol. 7, Issue 1, pp. 12343, (2017) (PubMed).

Comps-Agrar, Kniazeff, Nørskov-Lauritsen, Maurel, Gassmann, Gregor, Prézeau, Bettler, Durroux, Trinquet, Pin: "The oligomeric state sets GABA(B) receptor signalling efficacy." in: The EMBO journal, Vol. 30, Issue 12, pp. 2336-49, (2011) (PubMed).

Chacko, Liberante, Paul, Longley, Fennell: "Voltage dependent anion channel-1 regulates death receptor mediated apoptosis by enabling cleavage of caspase-8." in: BMC cancer, Vol. 10, pp. 380, (2011) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody