Goat anti-Monkey IgM Antibody (DyLight 800)

Catalog No. ABIN6698970

Product Details

Target: IgM

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 800

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), FLISA, Fluorescence Microscopy (FM)

Purpose: Monkey IgM (mu chain) Antibody DyLight™800 Conjugated

Characteristics: Goat Anti-Monkey IgM Antibody DyLight™ 800 Conjugation, Goat Anti-Monkey IgM DyLight™800 Conjugated Antibody,Anti-Monkey IgM (mu chain) antibody generated in goat detects specifically monkey IgM.

Immunogen: Monkey IgM whole molecule

Isotype: IgG

Labeling Ratio: 2.6

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Comment:

Anti-Monkey IgM (mu chain) DyLight™800 has been tested by dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.