Goat anti-Human IgM Antibody (DyLight 649)
(1 reference)
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- Target See all IgM products
- IgM
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- DyLight 649
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 609-143-007
- Supplier
- Rockland
- Purpose
- Human IgM (mu chain) Antibody DyLight™ 649 Conjugated
- Characteristics
- Goat Anti-Human IgM (mu chain) Antibody DyLight™ 649 Conjugated, Goat Anti Human IgM (mu chain) Antibody DyLight™ 649 Conjugated,Anti-Human IgM (mu heavy chain) DyLight 649 generated in goat detects specifically Human IgM mu heavy chain.
- Immunogen
- Human IgM whole molecule
- Isotype
- IgG
- Labeling Ratio
- 1.8
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Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 HRP
Goat anti-Human IgM antibody (HRP) - PreadsorbedELISA, FLISA Polyclonal IgG HRP
Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgM antibody (HRP)ELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Mouse anti-Human IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Monoclonal SA-DA4 IgG1 HRP
Mouse anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Monoclonal SA-DA4 IgG1 Alkaline Phosphatase (AP)
Goat anti-Human IgM antibodyELISA, IHC, WB Polyclonal IgG unconjugated
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- Application Notes
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- Comment
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The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Human IgM (mu heavy chain) DyLight 649 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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: "Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates." in: EBioMedicine, Vol. 99, pp. 104897, (2024) (PubMed).
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: "Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates." in: EBioMedicine, Vol. 99, pp. 104897, (2024) (PubMed).
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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