Goat anti-Human IgA (Heavy Chain) Antibody (DyLight 800)

Catalog No. ABIN6698907

Supplier Product No.: 609-145-006

Product Details

Target: IgA

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 800

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 609-145-006

Supplier: Rockland

Purpose: Human IgA (alpha chain) Antibody DyLight™800 Conjugated

Characteristics: Goat Anti Human IgA (alpha chain) Antibody DyLight 800™Conjugated, Goat Anti-Human IgA (alpha chain) Antibody DyLight 800™Conjugated, Goat Anti Human IgA Antibody DyLight 800™Conjugated,Anti-Human IgA DyLight 800 Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Immunogen: Human IgA alpha heavy chain

Isotype: IgG

Labeling Ratio: 2.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:80,000

Immunohistochemistry_Dilution: 1:1,000 - 1:5,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Comment:

Anti-Human IgA DyLight 800 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: Microarray

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

N'Guessan, Kailasam, Mostefai, Poujol, Grenier, Ismailova, Contini, De Palma, Haber, Stadler, Bourque, Hussin, Shapiro, Fritz, Piccirillo: "Selection for immune evasion in SARS-CoV-2 revealed by high-resolution epitope mapping and sequence analysis." in: iScience, Vol. 26, Issue 8, pp. 107394, (2023) (PubMed).

Fathi, Dahlke, Krähling, Kupke, Okba, Raadsen, Heidepriem, Müller, Paris, Lassen, Klüver, Volz, Koch, Ly, Friedrich, Fux, Tscherne, Kalodimou, Schmiedel, Corman, Hesterkamp, Drosten, Loeffler, Haagmans, Sutter, Becker, Addo: "Increased neutralization and IgG epitope identification after MVA-MERS-S booster vaccination against Middle East respiratory syndrome." in: Nature communications, Vol. 13, Issue 1, pp. 4182, (2022) (PubMed).

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody