Goat anti-Armenian Hamster IgG Antibody (DyLight 405) - Preadsorbed

Catalog No. ABIN6698889

Product Details

Target: IgG

Reactivity: Golden Syrian Hamster, Armenian Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 405

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Purpose: Golden Syrian & Armenian Hamster IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Mouse and Rat Serum Proteins

Characteristics: goat anti-Golden Syrian & Armenian Hamster IgG DyLight™405 conjugated antibody, goat anti-Hamster IgG DyLight™ 405 conjugated antibody,Anti-Golden Syrian & Armenian Hamster IgG DyLight Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule

Isotype: IgG

Labeling Ratio: 2.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: FC

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Hirve, Levytskyy, Rigaud, Guimond, Zal, Sauer, Tsoukas: "A conserved motif in the ITK PH-domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions." in: PLoS ONE, Vol. 7, Issue 9, pp. e45158, (2013) (PubMed).

Levytskyy, Hirve, Guimond, Min, Andreotti, Tsoukas: "In Vivo Consequences of Disrupting SH3-Mediated Interactions of the Inducible T-Cell Kinase." in: Journal of signal transduction, Vol. 2012, pp. 694386, (2012) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.