Donkey anti-Goat IgG Antibody (DyLight 649) - Preadsorbed

Catalog No. ABIN6698870

Product Details

Target: IgG

Reactivity: Goat

Host: Donkey

Clonality: Polyclonal

Conjugate: DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Characteristics:

Synonyms: Donkey anti-Goat IgG Antibody DyLight™ 649 Conjugated Pre-Adsorbed, Donkey anti-Goat IgG DyLight™ 649 Conjugated Antibody

Background: Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Goat IgG whole molecule

Isotype: IgG

Labeling Ratio: 3.2

Application Details

Application Notes:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Malykhina, Qin, Lei, Pan, Greenwood-Van Meerveld, Foreman: "Differential effects of intravesical resiniferatoxin on excitability of bladder spinal neurons upon colon-bladder cross-sensitization." in: Brain research, Vol. 1491, pp. 213-24, (2013) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody