Donkey anti-Goat IgG Antibody (DyLight 488) - Preadsorbed

Catalog No. ABIN6698858

Supplier Product No.: 605-741-125

Product Details

Target: IgG

Reactivity: Goat

Host: Donkey

Clonality: Polyclonal

Conjugate: DyLight 488

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 605-741-125

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins

Characteristics: donkey anti-Goat IgG Antibody DyLight™ 488 Conjugation, donkey anti-Goat IgG DyLight™ 488 Conjugated Antibody,Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Goat IgG whole molecule

Isotype: IgG

Labeling Ratio: 5.6

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Goat IgG DyLight 488 Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Onoda, Takeda, Umezawa: "Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle." in: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 4, (2017) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles." in: Frontiers in cellular neuroscience, Vol. 11, pp. 92, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.