Goat anti-Chicken IgG Antibody (DyLight 405) - Preadsorbed

Catalog No. ABIN6698806

Supplier Product No.: 603-146-126

Product Details

Target: IgG

Reactivity: Chicken

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 405

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 603-146-126

Supplier: Rockland

Purpose: Chicken IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins

Characteristics: goat anti-Chicken IgG DyLight™405 Conjugated Antibody, goat anti-Chicken IgG Antibody DyLight™405 Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY DyLight™405,Anti-Chicken IgG DyLight Antibody generated in goat detects chicken IgY.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Chicken IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF, Multiplex

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Göttle, Förster, Gruchot, Kremer, Hartung, Perron, Küry: "Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination." in: Glia, Vol. 67, Issue 1, pp. 160-170, (2019) (PubMed).

Göttle, Manousi, Kremer, Reiche, Hartung, Küry: "Teriflunomide promotes oligodendroglial differentiation and myelination." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 76, (2019) (PubMed).

Göttle, Sabo, Heinen, Venables, Torres, Tzekova, Parras, Kremer, Hartung, Cate, Küry: "Oligodendroglial maturation is dependent on intracellular protein shuttling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 3, pp. 906-19, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.