Mouse anti-Human lambda Light Chain (Lambda-IgLC) Antibody (CF®647)
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- Target See all Lambda-IgLC products
- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- CF®647
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Human Lambda Light Chain (HP6054), CF647 Conjugate
- Characteristics
- This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Purified human IgG myeloma proteins coupled to polyaminostyren microbeads
- Clone
- HP6054
- Isotype
- IgG2a kappa
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- Application Notes
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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293T, Raji or hPBL cells. Tonsil or Spleen
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
- Alternative Name
- Lambda Light Chain (Lambda-IgLC Products)
- Background
- Bence Jones Protein, BJP, IGLC 1/2/3, Mcg Marker, Paraprotein
- Molecular Weight
- ~22.5 kDa
- Gene ID
- 3535, 3546, 449585
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