Goat anti-Human IgM (Chain mu) Antibody (DyLight 594)

Catalog No. ABIN612132

Product Details

Target: IgM

Binding Specificity: Chain mu

Reactivity: Human

Host: Goat

Conjugate: DyLight 594

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Goat anti-human IgM ( chain) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.

Purification: Affinity purified using solid phase hamster IgG (H&L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified human IgM (mu chain)

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

References

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, (1993) (PubMed).

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody