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Goat anti-Human IgA (Chain alpha) Antibody (DyLight 800)

FACS, IF DyLight 800

Catalog No. ABIN611979

Datasheet as PDF

Target See all IgA products

IgA
Binding Specificity
103

61

40

18

15

13

8

6

3

1

1

1

1

1

1

1

1
Chain alpha
Reactivity
180

62

42

16

14

13

12

12

11

10

8

6

6

5

4

3

3

2

1

1
Human
Host
200

104

49

17

14

6

2
Goat
Conjugate
91

55

49

41

24

19

9

6

6

6

6

5

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
DyLight 800
Application
183

131

122

105

101

91

59

47

40

34

28

23

23

18

18

17

17

13

7

7

4

3

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics

Goat anti-human IgA (alpha chain) - Affinity Pure, DyLight 800 Conjugate.
Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm).
Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.

Purification

Affinity purified using solid phase mouse IgG (H&L)

Purity

> 95 % based on SDS-PAGE

Immunogen

Purified human IgA, (alpha chain)
Goat anti-Human IgA antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Human IgA antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Human IgA (Heavy Chain) antibody (FITC) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG FITC

Goat anti-Human IgA (Heavy Chain) antibody (Biotin)
ELISA, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgA (Heavy Chain) antibody (FITC)
ELISA, FACS Polyclonal IgG FITC

Goat anti-Human IgA (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgA antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgA antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human IgA (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Rabbit anti-Human IgA (Heavy Chain) antibody (FITC) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG FITC

Application Notes

This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions

For Research Use only
Format

Lyophilized

Concentration

1.0 mg/mL

Buffer

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative

Sodium azide

Precaution of Use

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice

Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage

4 °C
Target

IgA

Abstract

IgA Products

Target Type

Antibody