Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (DyLight 633)

Catalog No. ABIN611866

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Conjugate: DyLight 633

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight 633 Conjugate.
Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm).
Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.

Purification: Affinity purified using solid phase bovine IgG (H&L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Sheep IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment:

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

References

Kudla, Montessuit, Eskes, Berrier, Martinou, Ghazi, Antonsson: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, (2000) (PubMed).

Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, (2000) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody